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KMID : 1003820130170030263
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Journal Of The Korea Institute Of Oriental Medical Diagnostics
2013 Volume.17 No. 3 p.263 ~ p.273
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Anti-Inflammatory Effect of Extracts from Ligustrum obtusifolium S. fruits in RAW 264.7 Macrophages
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Moon Ju-Ho
Ko Heung
Shin Seon-Mi
Kim Ki-Tae
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Abstract
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Objectives: This study was designed to investigate the anti-inflammatory effect of extracts from Ligustrum obtusifolium S. fruits(LOF) in RAW 264.7 Macrophages stimulated with lipopolysaccharide(LPS).
Methods: We examined productions of nitric oxide(NO), reactive oxygen species(ROS), inducible isoforms of NO synthase(iNOS), cyclooxygenase-2(COX-2) to investigate the anti-inflammatory effect of LOF extracts. In addition, we measured generation of pro-inflammatory cytokines(TNF-alpha, IL-6).
Results: Cell viability showed that LOF extracts had no cytotoxicity in Raw 264.7 cells. The treatment with LOF extracts significantly decreased the generation of NO and pro-inflammatory cytokines(TNF-alpha, IL-6) in LPS-stimulated macrophage cells. Furthermore LOF extracts inhibited intracellular ROS generation dose dependently and reduced the expression of iNOS, COX-2 proteins.
Conclusions: These results showed that the LOF extracts had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells. These findings provide scientific support for the use of this Ligustrum obtusifolium S. for inflammatory-related diseases.
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KEYWORD
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anti-inflammation , Ligustrum obtusifolium S. , nitric oxide , nitric oxide synthase , reactive oxygen species (ROS)
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